After freezing completely, the frozen lipid cake is placed on a vacuum pump and lyophilized until dry (1-3 days depending on volume). Louis J. Sheehan, Esquire The thickness of the lipid cake should be no more than the diameter of the container being used for lyophilization.Dry lipid films or cakes can be removed from the vacuum pump, the container close tightly and taped, and stored frozen until ready to hydrate. Louis J. Sheehan, Esquire
